高氧胁迫下Peroxiredoxin2蛋白的细胞保护作用的研究

Cytoprotection Research of Peroxiredoxin 2 Protein under Oxidative Stress

衰老和凋亡是细胞的两个重要生理过程,一直以来都是细胞生物学领域研究的热点。Peroxiredoxin 2(Prdx2)蛋白是过氧化物酶的其中一个亚型,分布于细胞质中。为了研究它在高氧条件诱导的细胞衰老及凋亡中的保护作用,我们分别将大鼠来源的Prdx2基因转染进人间充质干细胞(Human mesenchymal stem cells,hMSCs)和HEK293T细胞中,并建立了稳定表达Prdx2蛋白的HEK293T细胞系,利用SA-β-gal染色(Senescence-Associated β-Galactosidase Assay)、TUNEL染色及磷酸化p53蛋白的免疫印迹来检测高氧处理后细胞的衰老和凋亡情况。实验结果表明,高氧处理细胞后,转染了Prdx2的hMSCs和HEK293T细胞其衰老和凋亡率与对照组相比都有较为明显的减少,暗示Prdx2蛋白在细胞抵抗氧化损伤中发挥了重要作用。

Senescence and apoptosis are two important physiological processes which are always studied most in cell biology field.Peroxiredoxin 2（Prdx2） protein is one isoform of peroxidases,distributing in cytoplasm.For studying its cytoprotection in senescence and apoptosis processes under hyperoxia stress,we separately transfected human mesenchymal stem cells（hMSCs） and HEK293T cells with SD rat-derived Prdx2,established HEK293T stable cell lines which can stably express Prdx2 protein.Then we utilized Senescence-Associated β-Galactosidase Assay,TUNEL staining and phosphorylated p53 protein western blot assay to detect celluar senescence and apoptosis after hyperoxia treatment.The results showed that both celluar senescence and apoptosis rates of hMSCs and HEK293T cells transfected with Prdx2 are apparently decreased,compared with control.It suggested that Prdx2 protein played an important role in protecting cells from oxidative damage.