摘要
目的:探讨葛根芩连汤对UC大鼠血清Bcl-2、TGF-β1的影响。方法:根据体重将60只SD大鼠随机分为正常组、模型组、对照药组、葛根芩连汤组;采用免疫诱导法(第1、10、17、24d于大鼠双侧足趾、双侧腹股沟及背部皮下注射抗原乳化液)复制UC大鼠模型;第30d观察大鼠结肠黏膜组织损伤程度并评分,ELISA法分析血清Bcl-2、TGF-β1水平变化。结果:UC模型大鼠结肠溃疡形成,可伴有炎症、充血、水肿等病理改变;UC模型大鼠血清Bcl-2、TGF-β1水平显著降低(P<0·05);葛根芩连汤组血清Bcl-2、TGF-β1升高(P<0·05)。结论:UC模型大鼠结肠黏膜损伤,葛根芩连汤可改善其结肠黏膜损伤程度,这可能与血清Bcl-2、TGF-β1水平的变化有关。
Objective： The study was designed to discuss the effect of GegenQinlian decoction on serum Bcl-2, TGF-β1 in ulcerative colitis. Methods： 60SD rats were randomly divided into control group, model group, control drug group and Gegen- Qinlian decoction group according to weight. The UC rats were induced by immunization （ antigen emulsion was injected in bilateral toes, bilateral inguinal an subcutaneous of rats at 1, 10, 17, 24day）. The colonic damage and score were administrated at 30d and serum Bcl-2and TGF-β1 were analyzed by ELISA. Results： The colonic ulceration might be associated with inflammation congestion, edema and other pathological changes in UC rats. The serum Bel-2and TGF-β1were significantly decreased in UC rats （P〈0. 05）, GegenQinlian decoction elevated the serum Bcl-2and TGF-β1in UC rats （P〈0.05）. Conclusion： Colonic mucosal injury in UC rats, C, egenQinlian decoction can improve the level of the colon mucosa, which may be concerned with the change of serum Bcl-2, TGF-β1.
出处
《四川中医》
2011年第11期12-14,共3页
Journal of Sichuan of Traditional Chinese Medicine
基金
辽宁卫生职业技术学院重点项目(编号:2009Z08)
