摘要
It is essential to predict the treatment efficacy of pancreatic carcinoma early.The purpose of this study was to examine whether ^(18)F-FDG(2'-deoxy-2'-[^(18)F]fiuoro-D-glucose) or ^(18)F-FLT(3'-deoxy-3'-^(18)F-fluorothymidine) PET can be used for chemosensitivity testing by investigating the binding characteristic of ^(18)F-FDG or ^(18)F-FLT with Patu 8988 human pancreatic carcinoma cell and the influence of gemcitabine in the uptake of ^(18)F-FDG or ^(18)F-FLT on Patu 8988.Under the conditions of 1×10~6 cells,3.7 kBq ^(18)F-FDG or ^(18)F-FLT,and incubation at 37℃for 100 min,the cell uptake of ^(18)F-FDG and ^(18)F-FLT was(60.60±3.05)%and(50.57±2.81)%,respectively.There was a significant decrease in TKl-LI(thymidine kinase 1 labeling index) 24 h after administration of gemcitabine.The uptakes of ^(18)F-FDG and ^(18)F-FLT were negatively correlated with the doses of gemcitabine(r= -0.928 for ^(18)F-FDG,r= -0.876 for ^(18)F-FLT,P<0.01).When same doses of gemcitabine were administered,the ^(18)F-FLT uptake inhibition rate was significantly higher than that of ^(18)F-FDG(P<0.01).These results indicate that the response to gemcitabine could be predicted as early as 24 h by ^(18)F-FDG or ^(18)F-FLT PET scans.^(18)F-FLT is more sensitive than ^(18)F-FDG to predict the response to therapy.
It is essential to predict the treatment efficacy of pancreatic carcinoma early. The purpose of this study was to examine whether ^18 F-FDG （2＇-deoxy-2＇-[^18F]fluoro-D-glucose） or ^18F-FLT （3＇-deoxy-3＇-^18 F-fluorothymidine） PET can be used for chemosensitivity testing by investigating the binding characteristic of ^18F-FDG or ^18F-FLT with Patu 8988 human pancreatic carcinoma cell and the influence of gemcitabine in the uptake of ^18F-FDG or ^18F-FLT on Patu 8988. Under the conditions of 1 × 10^6 cells, 3.7 kBq ^18F-FDG or ^18F-FLT, and incubation at 37℃ for 100 rain, the cell uptake of 18F-FDG and 18F-FLT was （60.60±3.05）% and （50.57±2.81）%, respectively. There was a significant decrease in TK1-LI （thymidine kinase 1 labeling index） 24 h after administration of gemcitabine. The uptakes of 18F-FDG and ^18F-FLT were negatively correlated with the doses of gemcitabine （r= -0.928 for ^18F-FDG, r= -0.876 for ^18 F-FLT, P〈0.01）. When same doses of gemcitabine were administered, the ^18F-FLT uptake inhibition rate was significantly higher than that of ^18F-FDG （P〈0.01）. These results indicate that the response to gemcitabine could be predicted as early as 24 h by ^18F-FDG or ^18F-FLT PET scans. ^18F-FLT is more sensitive than ^18F-FDG to predict the response to therapy.
基金
Supported by Key Laboratory Program of Nuclear Medicine,Ministry of Health of China(Grant No.KF200904)
