微波辅助提取明月草叶查尔酮工艺研究

Study on the Microwave-assistant Extraction of Chalcones from Angelica keiskei Leaves

对微波辅助提取明月草叶查尔酮的工艺进行了研究。在微波功率320 W条件下研究了乙醇浓度、液料比、提取时间和提取次数对明月草叶查尔酮提取量的影响,最优条件为乙醇体积浓度70%,液料比40︰1(mL/g),提取时间2 min,提取3次。最优条件下,提取物中查尔酮含量为1.039%。对明月草叶查尔酮粗提液进行清除羟基自由基(-OH)研究,结果表明其对羟基自由基具有较好的清除作用,10 mg/mL明月草叶查尔酮粗提液对羟基自由基清除率达到87.04%。

The microwave-assistant extraction of chalcones from the leaves of Angelica keiskei（LAK） was studied.The microwave power was controlled at 320 W,the effects of ethanol concentration,the ratio of extractant volume to LAK weight,microwave treating time and extraction times on the extraction yield of chalcones were studied.The results showed that the optimum extraction conditions were as follows： the concentration of ethanol was 70%（v/v）,the ratio of extractant volume to LAK weight was 40︰1（mL/g）,the extraction time was 2 min and the extract 3 times.Under these conditions the yield of chalcones was 1.039%.The extract isolated from LAK had good scavenging activities on hydroxyl radical（-OH）.The scavenging percentage of hydroxyl radical reached 87.03% when the concentration of the LAK extract was 10 mg/mL.