响应面法优化微波辅助提取山楂核三萜的研究

Optimization of Microwave-assisted Extraction of Triterpenoid in Hawthorn Seed by Response Surface Methodology

利用响应面分析法优山楂核三萜的提取工艺。以山楂核三萜的得率为考核指标,研究甲醇体积分数、液料比、微波功率、微波时间对山楂核三萜提取的影响,在单因素试验的基础上,根据Box-Benhnken设计原理,设计四因素三水平响应面分析法,建立二次多项式回归方程的预测模型,获得最佳工艺参数:液料比17 mL/g、甲醇体积分数60%、微波功率640 W、微波时间97 s。在此条件下获得山楂核三萜的理论得率为1.12%,实际测得山楂核三萜得率为(1.143±0.008)%,与理论预测值基本相符。

The optimal conditions for hawthorn seed were evaluated by means of response surface methodology.Microwave-assisted extraction was applied to extract triterpenoid from hawthorn seed.The effects of four independents variables in terms of methanol concentration,ratio of solvent-to-solid,microwave power and microwave processing time on the triterpenoid yield were determined.On the basis of single-factor experiments,a quadratic polynomial regression equation of the forecasting model was set up by using Box-Behnken design.The optimal extraction parameters to obtain the highest triterpenoid yield were ratio of solvent-to-solid 17 mL/g,methanol concentration 60%,microwave treatment time 97 s,microwave power 640 W.The average experimental triterpenoid yield under the optimum conditions was found to（1.143±0.008）%,which agreed with the predicted value of 1.12%.