抗精神病药对精神分裂症患者血小板腺苷A2a受体基因表达的影响

Effects of antipsychotics on platelet adenosine A2a receptor gene expression in un.medicated patients with schizophrenia

目的探讨未用药精神分裂症患者治疗前后血小板腺苷A2a受体（ADA2aR）mRNA表达量的变化及其与临床症状的相关性。方法采用实时定量聚合酶链反应技术，对30例未用药精神分裂症患者（患者组）治疗前和治疗6周后进行血小板ADA2aR mRNA表达量测定，并与30名健康志愿者（对照组）进行比较；采用Pearson相关分析对患者组血小板ADA2aR mRNA表达量与精神分裂症临床症状进行相关性分析。结果（1）治疗前，患者组血小板ADA2aR mRNA表达量（747．6±282．3）与对照组（692．7±286．7）比较，差异无统计学意义（P〉0．05）；治疗第6周末，患者组血小板ADA2aR mRNA表达量（873．2±206．2）高于对照组（635．4±263．2）及患者组治疗前，差异均有统计学意义（P〈0．05）。（2）患者组治疗前血小板ADA2aR mRNA表达量与病程（r：0．17）、PANSS总分（r=0．08）、阳性症状（r=-0．12）、阴性症状（r=0．08）、一般病理症状（r=0．12）均无统计学相关性（P〉0．05）；治疗第6周末，患者组血小板ADA2aR mRNA表达量与病程PANSS总分（r=0．09）、阳性症状（r=-0．02）、阴性症状（r=0．26）、一般病理症状（r=-0．08）亦无统计学相关性（P〉0．05）；患者组治疗前后血小板ADA2aR mRNA表达量差值与PANSS总分减分值（r=-0．31）、阳性症状减分值（r=-0．28）、阴性症状减分值（r=-0．14）、一般病理症状减分值（r=-0．11）均无统计学相关性（P〉0．05）。结论抗精神病药治疗可能对精神分裂症患者外周血小板ADA2aR基因表达产生影响，血小板ADA2aR基因表达变化可能与精神分裂症临床症状无明显联系。

Objective To explore the effects of antipsychotics on peripheric platelet mRNA level of adenosine A2a receptor （ADA2aR） in un-medicated patients with schizophrenia. Methods The mRNA level of ADA2aR in 30 un-medicated patients with schizophrenia was evaluated with real-time polymerase chain reaction （RT-PCR） at baseline and after six-week antipsychotic treatment, and 30 healthy volunteers were examined as controls. Results There were no significant difference （ P 〉 0. 05 ） in the mRNA level of ADA2aR between schizophrenics （747.6 ±282. 3 ） and controls （692. 7 ± 286. 7 ） at baseline. There were also no significant correlation between mRNA level of ADA2aR and scores of Positive and Negative Symptoms Scales （ PANSS ） （ P 〉 0. 05 ） at baseline. After six-week antipsychotic treatment, the mRNA level of ADA2aR in schizophrenics （ 873.2 ± 206. 2 ） were significantly higher than the baseline （ 747.6 ± 282. 3 ） and in controls （ 635.4 ± 263.2 ） （ all P 〈 0. 05 ）. No significant correlation between the mRNA level of ADA2aR and PANSS scores were found in schizophrenics and controls at baseline and endpoint （ P 〉 0. 05 ）. There were also no significant correlation between changes in the mRNA level of ADA2aR and PANSS scores （ P 〉 0. 05 ） in schizophrenics and controls. Conclusions The results suggested that antipsychotics treatment have effect on the gene expression level of adenosine A2a receptor on platelet and changes in gene expression level of adenosine A2a receptor could have no relationship with clinical symptom of schizophrenics.