C型产气荚膜梭菌α毒素基因核苷酸序列的测定与分析

Detection and Analysis of Nucleotide Sequence of Alpha-toxin Gene of Clostridium perfringens Type C

[目的]研究C型产气荚膜梭菌α毒素基因核苷酸序列的遗传变异特点。[方法]设计产气荚膜梭菌α毒素基因特异性引物进行PCR扩增,扩增产物纯化后测定核苷酸序列并与NCBI登录的参考序列通过DNAStar软件进行同源比对。[结果]序列分析表明,所测菌株α毒素基因核苷酸组成中腺苷酸含量较高,胞苷酸含量较低。所测菌株与13、C57-1、CER 89L43、S01、S08、T01以及T16菌株的核苷酸序列同源性分别为98.7%、98.7%、98.6%、98.8%、98.7%、97.0%、98.6%,推导的氨基酸序列同源性分别为98.6%、98.6%、98.9%、98.9%、98.9%、98.6%、98.6%。[结论]所测C型产气荚膜梭菌α毒素基因核苷酸序列及推导的氨基酸序列与参考序列同源性均较高。

[Objective]The aim was to study the genetic variation characteristic of nucleotide sequence of alpha-toxin gene within Clostridium perfringens type C.[Method]The special primers of alpha-toxin gene within Clostridium perfringens were designed for PCR amplification.Nucleotide sequence was detected after purification of the PCR products and was compared with the NCBI reference sequences by the DNAStar software.[Result]Sequence analysis indicated that adenosine was higher and cytidine was lower in the tested strain.The rate of nucleotide sequence homology between the tested strain and strain 13,C57-1,CER 89L43,S01,S08,T01,T16 was 98.7%,98.7%,98.6%,98.8%,98.7%,97.0%,98.6% and homology of the deduced a mino acid sequence was 98.6%,98.6%,98.9%,98.9%,98.9%,98.6%,98.6% respectively.[Conclusion]Nucleotide sequence of alpha-toxin gene within Clostridium perfringens type C and the deduced a mino acid sequence both had high homology with the reference sequences.