紫茎泽兰不同生长部位中β-香烯的含量测定

Content Determination of β-elemene in Different Parts of Eupatorium adenophorum Spreng

[目的]测定紫茎泽兰不同生长部位中抗肿瘤活性成分β-榄香烯的含量。[方法]采用HPLC法对攀西地区产的紫茎泽兰(Eupatorium adenophorum Spreng)不同生长部位中β-榄香烯的含量进行测定。色谱条件:色谱柱为Eclipse XDB-C18(4.6 mm×150 mm,5μm),流动相为乙醇-乙腈-水(70∶10∶20,V/V/V),流速为1.0 ml/min,检测波长为210 nm,进样量为20μl,柱温为30℃。[结果]紫茎泽兰植株各部位均含有活性成分β-榄香烯,且叶片中的含量最高。自然晒干叶中β-榄香烯含量为0.083%,比中药温莪术中的含量高3倍以上。[结论]从紫茎泽兰中提取β-榄香烯,不仅可大幅度降低β-榄香烯的原料成本,还可为我国恶性杂草植物的治理和开发利用开辟新的途径。

[Objective] The aim was to determine the contents of β-elemene in different parts of Eupatorium adenophorum Spreng.[Method] Contents of β-elemene in different parts of E.adenophorum grown in Panxi Area of China were measured by HPLC.The chromatographic conditions were as follows：Eclipse XDB-C18（4.6 mm × 150 mm,5 μm）,1.0 ml/min flow rate,210 nm detective wavelength,ethanol-acetonitrile-water as（70∶ 10∶ 20,V/V/V）mobile phase,20 μl injection volume,30 ℃column temperature.[Result] β-elemene existed in all parts of E.adenophorum plant.And the content was the maximum in leaves.In the naturally dry leaves,β-elemene content was 0.083%,which was more than 3 times higher than the content in Curcuma wenyujin Y.H.Chen.[Conclusion] Extracting β-elemene from E.adenophorum could greatly reduce the material cost of β-elemene,and could develop new methods for the control of malignant weeds in China.