低成本乳酸菌液配方筛选及其发酵效果研究

Study on the Formula Screening of Low-cost Lactobacillus Suspension and Its Fermentation Effects

[目的]研究能替代MRS生产乳酸菌的低成本培养基,为规模化生产乳酸菌发酵秸秆提供理论依据。[方法]在玉米面蒸煮液中加入不同浓度的葡萄糖、氯化钠接种乳酸菌发酵,测定其pH变化并与MRS培养基进行比较;选出最适合的低成本培养基生产乳酸菌并发酵秸秆,将其与未发酵秸秆同时进行黄牛饲养试验,研究各种饲料的消耗量。[结果]玉米面与MRS2种培养基中pH变化趋势相同,但玉米面培养基pH下降更为迅速;发酵秸秆饲料消化量大于未发酵饲料。[结论]玉米面培养基可以代替MRS生产乳酸菌,而且降低了80%以上的生产成本。

[Objective] The research aimed to study the low-cost medium instead of MRS to product lactobacillus suspension and provide theoretical basis for the large-scale production of stalk fermented by lactobacillus.[Method] Different concentrations of glucose and sodium chloride were added into the cooking liquor of maize flour,and lactobacillus was inoculated to ferment the stalk.The changes of pH was measured and compared with MRS medium.The optimum low-cost medium for producing lactobacillus and fermenting stalk was selected to ferment stalk.And the feeding experiment of cattle was made with fermented stalk and unfermented stalk to study the consumption quantity of different kinds of feeds.[Result] The changes of pH in cornmeal and MRS media had the same tendency,but the decrease of pH in cornmeal medium was faster.The consumed quantity of fermented stalk feed was larger than that of unfermented feeds.[Conclusion] Cornmeal medium can instead of MRS to produce lactobacillus and the production cost decreased more than 80%.