碱性蛋白酶水解螺旋藻蛋白的研究

Study on Enzymatic Hydrolysis to Spirulina Protein by Alcalase

[目的]研究碱性蛋白酶水解螺旋藻蛋白的工艺条件。[方法]采用L9(34)正交试验方法,研究了碱性蛋白酶对螺旋藻蛋白进行水解的最适温度、酶与底物的比(E/S)、pH等反应条件;并且以蛋白质的水解度为指标,优化出最佳工艺条件。[结果]在酶与底物比为5%、pH为8.5、温度为50℃的最佳工艺条件下,蛋白质水解度可达31.2%。[结论]该研究为螺旋藻进一步开发奠定了基础。

[Objective] The research aimed to study the best processing of alcalase hydrolyzed spirulina protein.[Method] The factors such as temperature,enzyme-substrate rate,pH value were optimized by mono-factor and orthogonal experiments.The optimal condition of the process was obtained by the degree of hydrolysis of protein.[Result] The results showed that the optimum reaction condition parameters were 5% enzyme-substrate rate,at 50 ℃ and pH 8.5.The degree of hydrolysis of protein could get 31.2% under the optimum reaction condition.[Conclusion] This research could provide scientific basis for the further exploit of Spirulina.