水分胁迫下不同油松种源SOD、POD、MDA及可溶性蛋白比较研究

A comparative study on the SOD,POD,MDA and dissoluble protein of six provenances of Chinese pine(Pinus tabulaeformis Carr.) under water stress

采用盆栽控水试验方法,设置正常水分（T1,田间持水量的70%~80%）、轻度胁迫（T2,田间持水量的50%~60%）和严重胁迫（T3,田间持水量的30%~40%）3个水分梯度,对6个油松Pinus tabulaeformis Carr.种源幼苗（陕西洛南LN,陕西桥山QO,山西灵空山LK,山西芦芽山LY,河北雾灵山WL和辽宁千山QN）的保护酶参数进行了分析。结果表明,在T3下,各种源的可溶性蛋白质含量、超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性和丙二醛（MDA）含量差异均极显著（P〈0.01）;LK种源的可溶性蛋白质含量显著高于其它种源,种源LK、WL和LN依靠提高可溶性蛋白质含量来调节细胞渗透势以抵抗干旱胁迫的能力较强;种源QN和QO的SOD活性达到最大,它们通过增加SOD活性来清除O2.-用以抵抗膜脂过氧化的能力较强。整个水分处理过程中,种源LN和LY的POD活性均较高,而QN的POD活性较低,LN和LY通过增加POD活性分解H2O2对细胞破坏的能力较强;各油松种源的膜脂质过氧化程度在T3下均有所降低。

Six provenances of Chinese pine（Pinus tabulaeformis Carr.）,including provenances Luonan（LN） and Qiao Mountain（QO） from Shaanxi,provenances Lingkong Mountain（LK） and Luya Mountain（LY） from Shanxi,provenance Qian Mountain（QN） from Liaoning and provenance Wuling Mountain（WL） from Hebei,were transplanted in pots in a greenhouse and treated with progressive water stress.The parameters of plant protective enzymes for them were measured under three soil water treatments（T1,normal water condition;T2,mild stress;and T3,severe stress;with 70%~80%,50%~60% and 30%~40% of the field capacity,respectively）.The results showed that there were significant differences（P0.01） in dissoluble protein content,superoxide dismutase activity（SOD）,peroxidase isozyme（POD） activity and molondialdehyde（MDA） content among these provenances under severe stress.Provenance LK had significant higher dissoluble protein content than the rest（P0.05）.Owing to higher dissoluble protein content,provenances LK,WL and LN had the ability of drought-resistance by increasing osmotic potential of the cell.Provenances QN and QO had the highest SOD activity under T3,and it indicated that these two provenances had the ability of resisting lipid peroxidation by increasing of the SOD activities and clearing O2？-.Under the water treatment from T1 to T3,provenances LN and LY had higher POD activity while provenance QN was lower than others.Provenances LN and LY have higher ability of resisting cell damage by eliminating H2O2.Membrane lipid peroxidation of all provenances is lower under T3.