骨骼肌收缩模式对p38/Akt磷酸化水平的影响

The influence of contraction modes on the phosphorylation of p38/Akt

目的:骨骼肌收缩可能通过非胰岛素依赖的途径促进葡萄糖摄取,而p38与Akt可能是其中起重要作用的分子。本文研究骨骼肌不同收缩模式对上述信号分子磷酸化的影响,从而探讨有效降低血糖的运动方式。方法:采用离体比目鱼肌肌条灌流技术及Western blot检测方法,研究不同模式的收缩对骨骼肌p38、Akt磷酸化水平的影响。结果:5 min 10%DC(duty cycle负荷率)和5 min 1%DC的收缩模式可分别使p38的磷酸化较对照组增加30%和34%,是激活p38的适宜刺激。但对Akt的磷酸化水平没有影响。结论:低强度有氧运动可以更好地激活p38,但不能有效激活Akt。

Objective： Muscle contraction may prompt glucose uptake through non-insulin-dependent ways, and it may be due to the enhanced activation of key proteins known to regulate glucose metabolism, like p38 and Akt. Our experiment focused on the impact of different contraction modes on the phosphorylation of the molecules, thus to explore effective ways to lower blood glucose. Methyls： Isolated muscle strips perfusion technique and Western blot analysis were employed to investigate the influence of different modes of contraction on the activation of the molecules. Results： Muscle contraction led to an increase in p38 phosphorylation, with the greatest effect observed after 5 minutes of 10% DC（duty cycle） contraction and 5 minutes of 1% DC contraction. However, phosphorylation of Akt were not altered by the two contraction modes. Conclusion： The level of phosphorylation of p38 was higher at the optimal contraction modes, but these modes could not increase the level of phosphorlation of Akt.