不同分子亚型乳腺癌中癌干细胞量的研究

Prognostic value of different amounts of cancer stem cells in different molecular subtypes of breast cancer

目的研究乳腺癌不同分子亚型中癌干细胞量的差别。方法根据分子亚型将乳腺癌组织标本分为A(乳腺腔内A型),B(乳腺腔内B型),C(HER-2过表达型),D(基底样型),E(正常细胞样型)5组,通过集落细胞法计数各组癌干细胞球,分析乳腺癌干细胞量与乳腺癌不同分子亚型的关系。结果 A,B两组的癌干细胞球数分别为(1.1±0.2)/1 000细胞和(1.3±0.1)/1 000细胞,组间差异无统计学意义(P>0.05);C组的癌干细胞球数为(8.6±1.0)/1 000细胞,明显多于A,B组(均P<0.05);D,E两组的癌干细胞球数分别为(22.4±1.2)/1 000细胞和(17.7±2.0)/1 000细胞,两组间差异无统计学意义(P>0.05),但均明显多于前3组(均P<0.05)。结论不同分子亚型乳腺癌组织中含有不同量的癌干细胞,对预测乳腺癌预后可能有一定意义。

Objective To investigate the differences in the amount of cancer stem cells in difirent breast cancer subtypes. Methods The specimens of breast cancer tissues were divided into five groups： group A （luminal A type）, B （luminal B type）, C （HER2 ＋ type）, D （basal-like type） and E （normal-like） according to their molecular subtypes. The cancer stem cell spheres in each group were measured through colony formation method, and then the relations of the amount of cancer stem eells with the subtypes of breast cancer were analyzed. Results The numbers of cancer stem cell spheres in group A and B were （ 1. 1 ± 0.2）/1 000 cells and （1. 3 ±0. 1 ）/1 000 cells, respectively, and this had no significant difference （P〉0.05）. The number of cancer stem cell spheres in group C was （8. 6 ±1.0）/1 000 cells, which was significantly higher than that in group A and B （ both P 〈 0. 05 ） . The numbers of cancer stem cell spheres in group D and E were （22. 4 ± 1. 2 ）/1 000 cells and （ 17. 7 ± 2. 0 ）/1 000 cells, respectively, and this was statistically similar （ P 〉 0.05 ） but either was significantly higher than that in the other three groups （ all P 〈 0. 05 ） . Conclusions Breast caucer tissues of different molecular subtypes contain varying amounts of cancer stem cells, and this may have certain significance for predicting the prognosis of breast cancer.