糖尿病大鼠精囊组织中神经生长因子和胆碱能M3受体的变化

Changes of nerve growth factor and M3 subtype muscarinic receptor in the seminal vesicle of diabetic rats

目的:观察糖尿病大鼠精囊组织中神经生长因子(NGF)和胆碱能毒蕈碱M3受体含量的变化,以探讨糖尿病自主神经病变对精囊的影响,为糖尿病不育的临床防治提供理论依据。方法:成年雄性普通级SD大鼠15只,随机分为正常对照组5只和糖尿病模型组10只。链脲佐菌素(STZ)制备糖尿病模型成功后饲养8周,取精囊组织进行苏木精-伊红(HE)染色和免疫组化染色。结果:与正常对照组相比,糖尿病模型组HE染色可见大鼠精囊平滑肌细胞减少,腺上皮胞质变薄,结构紊乱。免疫组织化学染色显示,糖尿病模型组NGF阳性强度明显增强,而M3受体则明显减弱,糖尿病模型组与正常对照两组间NGF和M3平均累积光密度(IA)[0.018 7±0.002 4 vs 0.010 0±0.001 5和0.020 9±0.008 5 vs 0.041 2±0.011 7]的差异均具有统计学意义(P均<0.01)。结论:NGF和M3受体在糖尿病鼠精囊中表达改变,提示糖尿病可致精囊自主神经病变。

Objective： To investigate the effect of diabetic autonomic neuropathy on the seminal vesicle and search for the theoretical evidence for the prevention and treatment of diabetic infertility by observing changes in the contents of the nerve growth factor（NGF） and muscarinic M3 receptor in the seminal vesicle of diabetic rats.Methods： Diabetic models were established in 10 of the 15 male adult SD rats by intraperitoneal injection of streptozotocin（STZ）,and the other 5 were included in a normal control group.Eight weeks after modeling,seminal vesicles were collected from the rats for HE and immunohistochemicl staining.Results： Compared with the normal controls,the diabetic models showed a decreased number of smooth muscle cells,thinner cytoplasm of glandular epithelial cells and disordered structure in the seminal vesicle.The intensity of NGF-positive staining was significantly enhanced,but that of M3 markedly reduced in the diabetic group.There were statistically significant differences in the mean integrated optical density（IA） of muscarinic M3 receptors and NGF between the control and diabetic groups（0.018 7±0.002 4 vs 0.010 0±0.001 5 and 0.020 9±0.008 5 vs 0.041 2±0.011 7,P0.01）.Conclusion： The changes in the expressions of NGF and M3 receptors in the seminal vesicle of diabetic rats suggest that diabetes mellitus may induce autonomic neuropathy of the seminal vesicle.