精液中性粒细胞弹性蛋白酶的研究进展

Advances in researches on polymorphonuclear neutrophil elastase in semen

影响男性生殖的因素很多,生殖道感染是其中重要因素之一。精液中性粒细胞弹性蛋白酶(polymor-phonuclear neutrophil elastase,PMNE)作为男性生殖道感染尤其是隐性感染诊断的重要炎症指标,对男性生育能力有潜在的负面影响。精液PMNE浓度不仅与精液白细胞数、精浆ROS水平显著相关,而且与其他炎症相关细胞因子如IL-6、IL-8、TNF-α水平等密切相关。PMNE对精子质量亦有影响,可以削弱精子活力,影响精子形态和DNA完整性等。精液中存在PMNE抑制剂,可与PMNE形成复合物,两者比例的失衡可以促进慢性炎症的发生,进而可能导致男性不育。检测精液PMNE的方法主要为酶免疫分析法,但检测方法仍需标准化,诊断标准尚需统一。

Reproductive tract infection is one of the important factors of male reproduction.Polymorphonuclear neutrophil elastase（PMNE） in semen,as a marker of male reproductive tract inflammation,especially recessive infection,potentially affects male fertility.The concentration of PMNE in semen is correlated significantly not only with semen white blood cell count and seminal plasma ROS level,but also with the levels of other inflammation related cytokines,such as IL-6,IL-8,and TNF-α.Furthermore,PMNE has a negative impact on sperm quality by decreasing sperm motility,increasing the percentage of morphologically abnormal sperm and interfering with DNA integrity.PMNE inhibitors in semen can form a compound with PMNE,and the imbalanced proportions of the two may promote the development of chronic inflammation,and consequently lead to male infertility.At present,PMNE in semen is detected mainly by enzyme immunoassay,but this method still needs to be standardized,and the diagnostic standards to be unified.