强精胶囊对精索静脉曲张大鼠附睾氧化/抗氧化系统的影响

Effects of Qiangjing Capsule on the oxidative and antioxidative system in the epididymis of varicocele rats

目的:观察强精胶囊对精索静脉曲张大鼠附睾氧化/抗氧化系统的影响,探讨其促进附睾精子成熟的可能作用机理。方法:100只青春期雄性SD大鼠,随机抽取10只为假手术组,其余90只以缩窄左肾静脉建立精索静脉曲张大鼠模型。术后4周证实有72只大鼠造模成功,并随机分为模型组、强精胶囊高剂量组、强精胶囊中剂量组、强精胶囊低剂量组、五子衍宗胶囊组和少腹逐瘀胶囊组,每组12只。分别给予生理盐水和不同浓度的强精胶囊(0.216、0.108、0.054 g/ml)、五子衍宗胶囊(0.130 g/ml)、少腹逐瘀胶囊(0.146 g/ml)混悬液按10 ml/kg灌胃,每日1次,共4周,检测大鼠左侧附睾谷胱甘肽过氧化物酶(GPx)活性与丙二醛(MDA)含量。结果:与假手术组比较,模型组GPx活性降低,MDA含量升高;强精胶囊高剂量组GPx活性、MDA含量较模型组、中、低剂量组、五子衍宗胶囊组、少腹逐瘀胶囊组差异有统计学意义(P<0.01,或P<0.05)。结论:精索静脉曲张可致大鼠附睾GPx活性降低,MDA含量升高;强精胶囊能一定程度提高GPx活性,降低MDA含量;并可能通过此环节,改善附睾微环境及其调控功能,促进精子成熟,提高生育力。

Objective： To observe the effects of Qiangjing Capsule（QC） on the oxidative amd antioxidative system in the epididymis of varicocele rats in comparison with those of Shaofuzhuyu Capsule（SC） and Wuziyanzong Capsule（WC）,and to explore its possible mechanism of enhancing epididymal sperm maturation.Methods： Ten of 100 adolescent male SD rats were randomized to a sham-operation group,and varicocele models were successfully established in 72 of the other 90 by narrowing of the left renal vein.Then the model rats were equally assigned to 6 groups： model control,high-dose QC（0.216 g/ml）,medium-dose QC（0.108 g/ml）,low-dose QC（0.054 g/ml）,SC（0.146 g/ml）,and WC（0.130 g/ml）.After 4 weeks of treatment,we determined the activity of glutathione peroxidase（GPx） and the level of malondialdehyde（MDA） in the left epididymis of different groups of rats.Results： Compared with the sham-operation group,the model group showed a significant decrease in GPx activity（P0.01） and a marked increase in the MDA level（P0.05）.And the high-dose QC group exhibited a significantly hither GPx activity and lower MDA level than all the other groups（P0.01 and P0.05）.Conclusion： Varicocele can reduce the activity of GPx and elevate the level of MDA in the epididymis of rats,while Qiangjing Capsule can increase the former and decrease the latter,and thereby may improve epididymal microenvironment,enhance epididymal sperm maturation and promote fertility.