三氯乙烯药疹样皮炎患者免疫相关基因的表达水平

Study on mRNA expression of immune-re lated genes in patients with allergic dermatitis induced by trichloroethylene

目的探讨三氯乙烯（TCE）药疹样皮炎患者外周血免疫相关基因Foxp3、GATA3、CTLA4、T—bet的mRNA表达。方法选取TCE药疹样皮炎患者8例为病例组，8例健康人为对照组，抽取两组对象的外周血，采用实时荧光定量PCR（real—timequantitativePCR）技术检测外周血免疫相关基因Foxp3、GATA3、CTLA4、T—bet的mRNA的表达水平。结果与对照组比较，病例组Foxp3、GATA3、CT—LA4基因mRNA表达水平分别升高115％、97％和241％，差异有统计学意义（P〈0．01）；与对照组比较，病例组T—bet基冈mRNA表达水平下降47％，差异有统计学意义（P〈O．01）。结论TCE药疹样皮炎的部分免疫相关基斟表达水平发生明显改变，这些基因可能在TCE引起的变态反应中发挥重要作用。

Objective To study mRNA expression of immune-related genes （Foxp3, GATA3, CTLA4 and T-bet） in peripheral blood of the patients with allergic dermatitis induced by trichloroethylene （TCE）. Methods The peripheral blood samples were collected from 8 healthy workers （control group） and 8 patients with allergic dermatitis induced by TCE （case group）. Real-time quantitative PCR was applied to detect mRNA expression of immune-related genes （Foxp3, GATA3, CTLA4, T-bet）. Results The mRNA expression levels of Foxp3, GATA3 and CTLA4 genes increased by 115%, 97% and 241% in case group, as compared with con-trol group （P〈0.01）. The mRNA expression level of T-bet gene decreased by 47% in case group, as compared with control group （P〈0.01）. Conclusion The mRNA expression levels of some immune-related genes changed in patients with allergic dermatitis induced by TCE, those genes may play an important role in TCE-induced allergy.