缺铜对大鼠自由基防御系统酶组分和血清溶血素的影响

EFFECTS OF DIETARY COPPER DEFICIENCY ON THEENZYMATIC COMPONENTS OF THE PRIMARYFREE RADICAL DEFENSE SYSTEMAND SERUM HEMOLYSININ RATS

初断孔Wistar大鼠分别饲以缺铜(Cu0.49mg/kg)和含铜(Cu 6.44mg/kg)饲料52至57天。测定大鼠组织中自由基防御系統酶组分[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSIHPx)]活性、脂质过氧化物(LPO)含量和血清溶血素含量等。与对照组相比,缺铜大鼠红细胞及各脏器CuZoSOD活性显著降低,CAT和GSHPx活性无明显变化,亦未观察到自由基引起组织损伤的产物LDO的升高。提示,缺铜时机体自由基防御系统酶组分确实受到了一定程度的影响,但机体对自由基损伤的防御能力,总体上没有明显受损。缺铜大鼠血清溶血素显著下降,说明块铜影响了机体的体液免疫功能。实验结果还提示,作为反映机体铜营养状况的指标,CuZnSOD比血红蛋白更敏感。

Weanling Wistar rats were fed a modified AIN-76 diet ad libitum for 25 to 57 days, either copper-deficient (0.49mg/kg; CuD) or copper-adequate (6.44mg/kg;CuA). The effects of dietary copper deficiency on the enzymatic components of the primary free radical defense system-super-oxide dismutase (SOD), catalasc (CAT) and glutathione peroxidase (GSH-Px), lipoperoxides (LPO) and serum hemolysin were examined. The amount of LPO exhibited no significant change even though SOD activity decreased significantly in the tissues of CuD rats, which indicated that Cu-ZnSOD activity responded directly to copper status of the body but the compromises in the overall protection against free radicals were not evident. The profound reduction in scrum hemolysin in CuD rats suggests that humoral immune function is copper dependent. The results also suggest that as an index of evaluating copper nutriture CuZnSOD is more sensitive than hemoglobin to copper deficiency.