载药聚乙烯醇/海藻酸钠静电纺丝纤维的改性及其药物体外释放研究

Study of modification of polyvinyl alcohol/sodium alginate electrospun fibers and its drug release ability in vitro

目的将聚乙烯醇(PVA)/海藻酸钠(SA)静电纺丝纤维进行改性,制备具有良好性能且能缓慢释放药物的伤口敷料。方法采用戊二醛蒸汽、3%氯化钙无水乙醇溶液和3%氯化钙饱和硼酸溶液对PVA/SA静电纺丝纤维进行交联后,以溶胀率为指标筛选出最佳的交联剂。以3%氯化钙无水乙醇溶液为交联剂,分别制备了交联时间为1、3和20 h的改性盐酸莫西沙星(MH)/PVA/SA静电纺丝纤维,然后将载药纤维于pH 7.4的PBS溶液中进行药物体外释放研究。结果通过筛选得知3%氯化钙无水乙醇溶液为最佳交联剂,其交联的纤维溶胀率为108%。在随后的MH/PVA/SA静电纺丝纤维药物体外释放实验中,我们发现随着交联时间的增长,药物释放越缓慢,在4 h时,未交联和交联1、3和20 h的累积释放率分别为66.63%、57.55%、40.90%和12.41%。结论采用3%氯化钙无水乙醇溶液为交联剂改性的PVA/SA静电纺丝纤维耐水和溶胀性能良好,将更适合于用作难愈合且有渗出液伤口的敷料。

Objective To prepare a kind of wound dressing that has good properties and can slowly release drugs using modified polyvinyl alcohol（PVA）/sodium alginate（SA） electrospun fibers.Methods After PVA/SA electrospun fibers were cross-linked by glutaraldehyde vapor,3% calcium chloride/ethanol solution and 3% calcium chloride/saturation boracic acid solution,respectively,the optimal cross-linker was screened out based on swelling ratio.Using 3% calcium chloride/ethanol solution as a cross-linker,1 h-,3 h-and 24 h-cross-linked moxifloxacin hydrochloride（MH）/PVA/SA electrospun fibers were modified,and then their drug release abilities in vitro were analyzed in PBS solution（pH 7.4）.Results The screening experiment showed that 3% calcium chloride/ethanol solution was the optimal cross-linker.The swelling ratio of the fibers modified with this cross-linker was 108%.In the following drug release test in vitro,we found that the drug could be released by MH/PVA/SA electrospun fibers more slowly with the increase of cross-linking time.The cumulative release rates of the 0-h-,1-h-,3-h-and 24-h-cross-linked fibers at 4 h were 66.63%,57.55%,40.90% and 12.41%,respectively.Conclusion The MH/PVA/SA electrospun fibers modified by 3% calcium chloride/ethanol solution has good water resistance and swelling property,and would be more suited as wound dressing for exudates-containing and difficult healing wounds.