基底细胞型乳腺癌患者肿瘤内树突状细胞呈HLA-DR抗原表达细胞的临床意义

Clinical Significance of Dentric Cells and HLA-DR Antigen Positive Cells in Tumor Tissue of Patients with Basal-like Breast Carcinoma

目的:观察基底细胞型乳腺癌患者肿瘤内树突状细胞、呈HLA-DR抗原表达细胞引起的抗肿瘤免疫并探讨其临床意义。方法:应用免疫组织化学二步法检测基底细胞型乳腺癌患者肿瘤组织内S-100蛋白阳性的树突状细胞及呈HLA-DR抗原阳性表达的细胞。结果:基底细胞型乳腺癌患者肿瘤组织内缺乏树突状细胞(2/22,占9.09%),数量明显少于非基底细胞型乳腺癌患者(138/138,占100%),癌细胞的HLA-DR抗原阳性表达(4/22,占18.1%)明显少于非基底细胞型乳腺癌(106/138,占76.8%)。结论:基底细胞型乳腺癌患者肿瘤组织缺乏由树突状细胞、淋巴细胞及HLA-DR抗原阳性表达的癌细胞引起的特异性抗肿瘤免疫。

Objective： To investigate the anti-tumor immunity conferred by S-100 protein positive dendritic cells （ DCs ） and HLA-DR antigen positive cells in tumor tissue from patients with basal-like breast carcinoma （ BLBC ）. Methods： The frequencies of dendritic cells expressing S-100 and HLA-DR antigen positive cells in BLBC tissues were determined using immunohistochemistry. Results： Both S-100＋ DCs and HLA-DR antigen positive cells were absent in most patients with BLBC （ 2/22, 9% ） and the total number ofDCs （ both S-100＋ and S-100- ） was significantly lower in BLBC patients that in controls （ 138/138, 100% ）. The HLA-DR＋ cancer cells were observed in significantly fewer BLBC patients （ 4/22, 18.1% ） than in control patients （ 106/138, 76.8% ）. Conclusion： The specific anti-tumor immunity brought about by the infiltration of DCs and DR＋ carcinoma cells was largely absent in the tumor tissues of BLBC patients, suggesting that the presence of S-100＋ DCs and HLA-DR antigen positive cells may be related to postoperative prognosis.