摘要
本文报告了富硒麦芽粉阻断黄曲霉毒素(AFB_1)诱导血白细胞DNA非程序合成(Unsheduled DNA Synthesis,UDS)的作用。 大鼠以富硒麦芽粉Se lppm饲养,可使外周血白细胞经AFB_1 6×10^(-7)M体外诱导的UDS值明显降低。大剂量AFB_1(1mg/kg)一次灌胃,或以每次250μg/kg,二周内10次灌胃的大鼠,如预先饲以富硒麦芽饲料,亦能明显降低其白细胞UDS值。 正常人每日食用300μg硒的硒麦芽粉强化饼干,一年后,可明显降低AFB_1所致人外周血UDS值,麦芽饼干组UDS值为2.56。而富硒麦芽饼干组UDS值为0.35。 本实验提示:富硒麦芽有阻断致癌物AFB_1对外周白细胞UDS的诱发作用。实验中富硒麦芽无毒性但具有硒的生物效应,可望在低硒或肝癌高发区作为防病防癌食品添加剂推广。
The blocking effect of selenium-enriched malt (Se-malt) on AFB1-in-duced UDS was reported. When the rats were fed with food containing lppm Se in form of Se-malt for two weeks, the UDS values of leukocytes treated with 6×10-7M AFB1 in vitro was decreased from 54.8 ±14.0 opm/106 WBC to 35.1±10.0 cpm/106 WBC (p<0.05). In the in vivo experiments, the rats were presupplemented with a Se.-malt (lppm Se) for two weeks and then gavaged with a single dosage of AFB1 (1mg/kg). It was found, under this condition, that the UDS value was reduced from 277.1±72.6 cp-m/106 WBC in the- control group to 141.6±43.6 cpm/106 WBC in the Se-malt group (p<0.05). If the rats were fed with AFB1 250ug/kg for the times during the period of two weeks, the UDS value in the Se-malt group (70.3±19.0 opm/106 WBC) was also significantly lower than that in the control (114.0±19.0 cpm/106 WBC, p<0.05).After daily supplementation of Se-malt containing 300 ug Se to men for one year, the ratio of UDS was decreased from 2.56±1.06 in the control group to 1.35±0.43 in the Se-malt group (p<0.05) and no adverse effect was observed. Thus the protection of Se-malt against the attacking effect of AFB1 on DNA was demonstrated both in animals and men.
出处
《营养学报》
CAS
CSCD
北大核心
1990年第3期248-252,共5页
Acta Nutrimenta Sinica
