摘要
目的建立丹参药材HPLC方法同时用于指纹图谱和多指标测定。方法采用Diamonsil C18柱(250mm×4.6mm,5μm),在280nm波长下,以乙腈-0.05%磷酸水溶液梯度洗脱,测定了19批丹参药材的指纹图谱和丹参素、迷迭香酸、丹酚酸B及丹参酮ⅡA4个指标成分,对指纹图谱共有峰和相似度进行分析,并进行聚类分析和主成分分析。结果在选定的色谱条件下,得到19批丹参药材的指纹图谱,标定了12个共有峰,并建立了丹参药材的指纹图谱共有模式,相似度在0.808~0.997。聚类分析结果将19批丹参药材分为两大类,与主成分分析结果及药材测定结果相一致。结论不同产地丹参药材色谱峰的数目差别不大,但各指标成分量差别较大。该方法准确、可靠,为有效地评价丹参药材的综合质量提供了参考依据。
Objective To establish an HPLC method for Salvia miltiorrhiza radix et rhizoma fingerprint and simultaneous determination. Methods The HPLC method was used with Diamonsil-C18 column (250 mm × 4.6mm,5μm),under the wavelength of 280 nm, a mixed liquid of acetonitrile-0.05% H3PO4 as mobile phase in a gradient elution.The common HPLC fingerprint for 19 batches of Salvia miltiorrhiza radix et rhizoma was established,the contents of propanoid acid, rosmarinic acid, salvianolic acid B,and tanshinone ⅡA were determined,and the common peak and the similarity were analysed with parameter of fingerprint analyses.Results Under the selected spectrum condition,it acquired the fingerprint for 19 batches of Salvia miltiorrhiza radix et rhizoma and 12 common peaks.The 19 batches of samples were classified into two groups based on the results of hierachical clustering analysis. It shows no difference with principle components analysis and the results of simultaneous determination.Conclusion The amounts of the peaks from different habitats were the same,but the contents of components showed significant difference in Salvia miltiorrhiza radix et rhizoma from different habitats.The method is accurate and reliable,and it could provide comprehensive reference for the quality evaluation of Salvia miltiorrhiza radix et rhizoma.
出处
《药物评价研究》
CAS
2011年第5期343-347,共5页
Drug Evaluation Research
基金
河北省中小企业创新基金项目
关键词
丹参
高效液相色谱法
指纹图谱
Salvia miltiorrhiza radix et rhizoma
HPLC
fingerprint