脑电监测SD大鼠异氟醚全身麻醉模型的建立及脑电分析

The Establishment of SD Rats with Dynamic EEG Monitoring Model Under General Anesthesia with Isoflurane

目的:建立脑电监测SD大鼠异氟醚全身麻醉模型并分析脑电监测结果。方法:随机选取SD大鼠20只,先行脑电电极置入术,术后使用密闭吸入麻醉动物行为学观察圆筒,观察异氟醚引起的麻醉诱导、维持、觉醒状态并记录诱导、觉醒时间。将记录的行为学结果对照典型脑电图波形改变判断麻醉深度。结果:实验SD大鼠均检测出脑电图,通过对照行为学观察发现动态脑电监测结果同异氟醚麻醉过程进展一致。在麻醉过程中SD大鼠出现典型的全身麻醉脑电循环。结论:动态脑电监测和SD大鼠行为学观察可以准确反应全身麻醉深度。

Objective： To creative a dynamic EEG monitoring SD rats under isoflurane-induced general anesthesia of making ani- mal models and analysis the result of EEG was monitoring. Methods： EEG electrodes were implanted in 20 SD randomly selected rats, and then observed their behavior from the sealed animal observation cylinder. The subtle presentations and precise time of ioflurane-in- duced anesthesia, maintenance, and wakefulness in general anesthesia were respectively recorded. The degree of anesthesia can be judged by comparing the record with the typical EEG waveforms. Results： The EEG waveforms could be detected form SD rats in the ex- periment. By using the comparison behavior method, the results of dynamic EEG monitoring were observed the same result of the pro- cess of isoflurane anesthesia. SD rats showed the typical anesthesia EEG cycle during the anesthesia. Conclusion： Through the compari- son of the animal behavior observation of SD rats with dynamic EEG monitoring results, the dynamic EEG monitoring could accurately reflect the general anesthesia degree.