头孢曲松神经保护作用的研究

The Protective Effect of Ceftriaxone on Nervous System

目的:探讨头孢曲松通过抑炎通路发挥神经保护作用的机制。方法:45只雄性SD大鼠,随机分为3组(n=15),预处理组在大鼠大脑中动脉阻断(MCAO)前5 d每日给予腹腔注射头孢曲松缓冲液(200 mg/kg),观察局灶性脑缺血2 h再灌注24 h后,各组间大鼠神经行为学评分、脑梗死容积变化、小胶质细胞和IL-1β的数量变化。结果:再灌注24 h后,头孢曲松预处理组可改善大鼠神经行为学评分和脑梗死面积(P<0.05),小胶质细胞活化数量减少(P<0.05),ELISA检测IL-1β分泌数量有显著下降趋势,但无统计学差异(P=0.18)。结论:头孢曲松可以部分抑制小胶质细胞活化,减少IL-1β释放发挥神经保护作用。

Objective： To investigate the mechanism of ceftriaxone in neuroprotecion. Methods： Ceflriaxone was given daily for five days before focal cerebral ischemia was induced by middle cerebral artery occlusion （MCAO） for 120 mins in male Sprague-Dawley rats. The neurobehavioral scores, infarct volumes, the amount change of microglia and interleukin-1β were detected. Results： Ceftriax- one can significantly improve the neurobehavioral score and infarct volumes at 24 h of reperfusion. The number of active microglia sig- nificantly decreased, the ELISA test result shows the decreasing of inerleukin-1β but there was no significant difference（p=0.18）. Conclusion： Ceftriaxone plays neuroprotective role by inhibiting the inflammation response.