武警某部新兵军事训练前后NK细胞活性和T细胞转化功能测定

Subject The detection of the recruit from the Chinese People＇s Armed Police Force on the NK activity and T lymphocytes transformation

目的 研究武警某部新兵军事训练前后NK细胞活性和T细胞转化功能的变化.方法 采用乳酸脱氢酶（LDH）释放实验,测定NK细胞杀伤活性.采用MTT比色法,测定T淋巴细胞的增殖反应.结果 军事训练第二个月（应激期）,新兵NK细胞杀伤活性和T细胞转化功能与训练前期（基础期）和训练期第三个月后（恢复期）相比较差异有统计学意义（P〈0.05）,军事训练应激期,新兵NK细胞杀伤活性和T细胞转化功能明显升高.结论 军事训练可改变新兵NK细胞活性和T细胞转化功能状态,从而调节机体免疫平衡.

Objective To study the changes before and after military exercise for the recruit on the NK activity and T lymphocytes transformation.Methods The NK cell activity was determined by lactate hydrogenase （LDH） elution method, and T lymphocytes transformation was measured with tetrazolium salt （MTT） assay. Results The NK activity and T lymphocytes transformation of the recruit at stress phase（the second month） showed clinically significant differences compared with fundamental phase and convalescence （P〈0.05）. Conclusion Military examination can affect the NK activity and T lymphocytes transformation of the recruit, which is conducive to retain immune balance.