催化反应液中氨基葡萄糖酸的光度法测定

New method for photometric determination of D-glucosaminic acid in catalytic reaction solution

利用氨基葡萄糖酸在强酸性(pH 1.5)条件下形成内酯,并与羟胺碱-三氯化铁显色体系生成红棕色络合物,建立了氨基葡萄糖酸的分光光度检测方法,给出了线性方程。检测波长为500 nm,在1.0×10-5～9.0×10-5 mol/mL范围内呈现良好的线性关系,平均加标回收率为99.28%,相对标准偏差≤4.91%。该方法操作简单、结果稳定、选择性和灵敏度良好,能成功应用于实际样品的检测。

Based on the ability of D-glucosaminic acid to form the corresponding lactone at pH 1.5 which can react with hydroxylamine base-ferric trichloride color system to form a red-brown complex, the spectrophotometric detection method is established. The maximum absorbance of the complex is at 500 rim. The corresponding linear equation is A = 0. 0926C ＋0. 018 ,R2 =0. 9901 ,ε =4. 683 × 10^4 L/（cm.mol）. The linear range is 1. 0 × 10^-5 -9. 0 ×10^-5 mol/mL and the average recovery 99.28%, RSD ≤4. 91%. The selectivity and sensitivity of this method are satisfactory and the results are stable. This method can be successfully applied to the detection of actual sample.