强化生物除磷工艺微生物种群结构分析

Analysis of microbial community structure in enhanced biological dephosphorization

引言 强化生物除磷工艺以其高效、低耗、简单易操作在世界范围内得到了广泛的应用，近年来反硝化生物强化除磷工艺一直是个研究热点，主要是由于反硝化生物强化除磷工艺可通过反硝化除磷菌（DPB）在传统的厌氧／缺氧／好氧工艺的缺氧池中利用内碳源聚β-羟基丁酸酯（PHB）作为碳源实现反硝化吸磷，同时亦可反硝化脱氮，“一碳两用”，可节省大量的碳源需求，同时也减少了一定的动力消耗和污泥产量，尤其适合低碳氮比的城市生活污水。

In order to study the microbial community structure and diversity as well as the performance of phosphorus removal associated with microbial community change, the fingerprint profile technique of PCR amplified 16S rRNA and denaturing gradient gel electrophoresis （DGGE） were applied to analyse the microbial communities in Bi-Bio-Selective simultaneous Phosphorus and Nitrogen removal （BBSPN） process. After the characteristic bands eluted from gel were further recovered, cloned （reamplified） and sequenced, the sequences were compared with those in GenBank database and used for homology analysis, and the phylogenetic trees were constructed. The sequence of DGGE bands obtained showed that there is a good dynamic correlation between microbial community variation and its removal performance of process. TP removal efficiency of BBSPN process was lower when a-proteobacteria was the dominant species, which mean a-proteobacteria was not favorable for enhanced biological phosphorus removal; whereas when Bacteroidetes was the dominant species, TP removal efficiency and denitrifying dephosphorization were better and stable, which should attribute to Uncultured Sphingobacteriaceae. So, the Uncultured Sphingobacteriaceae was like to be one of the denitrifying phosphate accumulating organisms （DPAOs） in this process. Moreover, Acinetobacter, TM7 and Uncultured Trichococcus are also beneficial for phosphorus and nitrogen removal of BBSNP process.