洛巴伊口蘑生长过程中几种胞外酶活性变化

Activity Changes of Several Extracellular Enzyme During the Growth Period of Tricholoma giganteum

为了解洛巴伊口蘑胞外酶的分泌特点,以菌株Tr0005和Tr0013为材料,采用分光光度法测定不同生长时期的9种胞外酶活性。结果表明:CMC酶、FP酶、β-葡萄糖苷酶、HC酶、果胶酶和蛋白酶活性高峰出现于子实体成熟期(分别达29U,4U,21U,196U,28U和40U以上);胞外淀粉酶活性在菌丝生长阶段较高(达10U以上),进入生理成熟期就持续下降;胞外漆酶和多酚氧化酶在菌丝生长阶段一直较高(分别达65U和54U以上),原基形成后逐渐下降。不同菌株只影响胞外酶活性的大小,但胞外酶活性变化规律是一致的。

In order to understand the secretion feature of Tricholoma giganteum,the strain Tr0005 and Tr0013 were determined their extracellular enzyme activity during the growth period by spectrophotometry.The activity peaks of extracellular CMCase,FPase,β-glucosidase,HCase,pectinase and protease were at the time when the fruit body matured （more than 29 U,4 U,21 U,196 U,28 U and 40 U）.The activity of extracellular amylase was at high level at the early stage of mycelium growth （more than 10 U）,and gradually declined during the stage of physiological maturity.The activities of extracellular laccase and polyphenol oxidase were at high level at the stage of mycelium growth （more than 65 U and 54 U）,and decreased gradually after primordium formation.Different strains can influence the magnitude of extracellular enzyme activity,but they had the same change law.