辐射诱导人肝细胞子代基因差异表达及生物信息学分析

Analysis of Differential Gene Expression Profile Induced by Ionizing Radiation in the Progeny of Normal Human Liver Cells

利用基因芯片技术和实时荧光定量RT-PCR技术,采用不同剂量60Coγ射线照射人正常肝细胞系HL-7702细胞,观察比较受照细胞克隆子代基因表达图谱的变化,筛选出差异表达基因,利用生物信息学方法分析构建基因相互作用网络图,对部分差异表达基因进行验证。结果表明,与对照组相比,共同的差异表达基因有71个,其中上调的35个,下调的36个,差异表达的基因数目与照射剂量有一定的相关性;对差异表达基因HAVCR2、RAN进行实时荧光PCR定量分析,与基因芯片结果一致;利用生物信息学分析软件构建了差异表达基因相互作用网络图并分析了RAN、CDT1等基因的生物学功能。结果提示,电离辐射可诱发HL-7702子代细胞中一系列基因的改变,生物信息学分析初步揭示了上述基因有可能成为辐射诱发基因组不稳定性的潜在分子生物学标志。

cDNA gene chip was used to measure the transcriptional profile in progeny of HL-7702 cells exposed to 0,2,4,and 6 Gy 60Co γ-irradiation.The experimental results show that a total of 71 differentially expressed genes were screened,most of which was associated with transduction,cell cycle regulation,cellular immunity,cytoskeleton and movement,cell replication and repair mechanism.A pathway-based network was constructed to reveal the biological functions of RAN,CDT1.Irradiation can induce differentially expressed genes in the progeny of irradiated cells,which may be applied as potential biomarkers of radiation damage in future studies.