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放射性工作人员外周血CDKN1A基因表达水平调查 被引量：1

A Pilot Study of Expression of CDKN1A Gene in Radiation Workers
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摘要 采用荧光实时定量RT-PCR技术,对某厂放射性工作人员外周血CDKN1A基因mRNA水平进行调查。结果表明,与对照组相比,放射性工作人员CDKN1A基因mRNA水平差异不显著;外周血白细胞数及分类差异不显著;CDKN1A基因表达水平Ct值约是-βactin的1.6倍。 The aim of the present study is to investigate the expression mode of CDKN1A gene in radiation workers preliminarily.The fluroescence quantitative real time RT-PCR was applied to quantify the level of the target gene in the leucocytes of peripheral blood cells.The results showed that no difference in the expression of CDKN1A gene was observed between the groups of radiation workers and the control.Furthermore,there was no change in the accounts and percentages of types of leucocytes.The Ct value of CDKN1A mRNA was 1.6 times that of beta-actin.The finding is useful in determination of the normal value of CDKN1A mRNA in healthy people.
作者 池翠萍 魏锦萍 郭凤玲 郭剑平 李曙芳
机构地区 中国辐射防护研究院
出处 《辐射防护通讯》 2011年第2期29-32,共4页 Radiation Protection Bulletin
关键词 CDKN1A基因 放射性工作人员 实时定量RT-PCR CDKN1A gene Radiation workers Real time quantitative RT-PCR
分类号 R14 [医药卫生—公共卫生与预防医学]
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参考文献12

  • 1Abbas T, Dutta A. p21 in Cancer: Intricate Networks and Multiple Activities. Nature Reviews. Cancer, 2009,9(6): 400.
  • 2Levine A J, Oren M. The First 30 Years of p53: Growing ever more Complex. Nature Reviews. Cancer, 2009, 9 (10) :749.
  • 3Takeuchi S, Takahashi A, Motoi N, et al. Intrinsic Coop- eration Between pl6INK4a and p21Wafl/Cipl in the Onset of Celhflar Senescence and Tumor Suppression in Vivo. Cancer Resem'ch, 2010,70(22) :9381.
  • 4Yu J H, Zhu B M, Wickre M, et al. The Transcription Factors Signal Transducer and Activator of Transcription 5A (STATSA) and STNF5B NegativeLy Regulate Cell Prolifera- tion through the Activation of Cyclin-dependent Kinase In- hibitor 2b (Cdkn2b) and Cdknla Expression. Hepatology, 2010,52(5) : 1808.
  • 5Cole A M, Ridgway R A, Derkits S E, et al. p21 Loss Blocks Senescence Following Apc Loss and Provokes Tu- mourigenesis in the Renal but not the Intestinal Epithelium. Nature Reviews. Cancer, 2010,2( 11 ) : 472.
  • 6George R J, Sturmoski M A, May R, et al. Loss of p21Wafl/Cipl/Sdil Enhances Intestinal Stem Cell Survival Following Radiation Injury. American Journal of Physiology. Gastrointestinal and Liver Physiology, 2009,296 (2) : G245.
  • 7池翠萍,李曙芳.^(137)Csγ射线慢性照射对CDKN1A基因表达的影响[J].辐射防护通讯,2010,30(5):22-26. 被引量：3
  • 8Turtoi A, Sharan R N, Srivastava A, et al. Proteomic and Genomic Modulations Induced by γ-irradiation of Htanan Blood Lymphocytes. International Journal of Radiation Biol- ogy, 2010,86(10) : 888.
  • 9Amundson S A, Do K T, Fomlace A J. Induction of Stress Genes by Low Does of Gmnrna Rays. Radiation Research, 1999,152(3) :225.
  • 10Bustin S A, Mueller R. Real-time Reverse Transcription PCR(qRT-PCR) and Its Potential Use in Clinical Diagno- sis. Clinical Science, 2005,109(4) : 365.

二级参考文献13

  • 1Monson R R,Cleaver J E,Abrams H L,et al.Health Riskes from Exposure to Low Levels of Ionizing Radiation:BEIR Ⅶ-phase 2.National Academies Press,2006.
  • 2Marchetti F,Coleman M A,Jones I M,et al.Candidate Protein Biodosimeters of Human Exposure to Ionizing Radiation.Int.J.Radiat.Biol.,2006,82(9):605.
  • 3UNSCEAR.Sources,Effects and Risk of Ionizing Radiation.UNSCEAR 1988 Report.
  • 4Amundson S A,Do K T,Formace A J.Induction of Stress Genes by Low Does of Gamma Rays.Radiat.Res.,1999,152(3):225.
  • 5Amunclson S A,Grace M B,Mcleland C B,et al.Human in Vivo Radition-induced Biomarkers:Gene Expression Changes in Radiotherapy Patients.Cancer Res.,2004,64(18):6368.
  • 6Amundson S A,Paul S.Development of Gene Expression Signatures for Practical Radition Biodosimetry.Int.J.Radiat.Oncol.Biol.Phys.,2008,71(4):1236.
  • 7Waters R L,Packard A T,Kramer G F,et al.Differential Gene Expression in Primary Human Skin Keratinocytes and Fibroblasts in Response to Ionizing Radiation.Radiat.Res.,2009,172(1):82.
  • 8Romanenko A,Lee C C,Yamamoto S,et al.Urinary Bladder Lesions after the Chernobyl Accident:Immunohistochemical Assessment of p53,Proliferating Cell Nuclear Antigen,Cyclin D1 and p21WAF1/Cipl.Jpn.J.Cancer Res.,1999,90(2):144.
  • 9Joyce N C,Harris D L.Decreasing Expression of the G1phase Inhibitors,p21Cipl and p16INK4a,Promotes Division of Corneal Endothelial Cells from Older Donors.Mol.Vis.,2010,16:897.
  • 10Hackl M,Brunner S,Fortschegger K,et al.miR-17,miR-19b,miR20a,and miR-106a are Down-regulated in Human Aging.Aging Cell,2010,9(2):291.

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辐射防护通讯
2011年 第2期

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