1.0 Gy ^(60)Coγ射线诱导人淋巴细胞基因表达的改变

Study of Gene Expression in Human Lymphocyte Exposed to 1.0 Gy ^(60)Co gamma ray

利用Human-6全基因组表达谱微珠芯片技术和实时荧光定量RT-PCR技术,采用60Coγ射线1.0 Gy剂量照射人离体外周血淋巴细胞,观察比较照后不同时间基因表达水平的改变,并对部分差异表达基因进行验证。结果表明:与对照组相比,照后6 h,差异表达基因有2 615条,其中上调的1 267条,下调的1 348条;照后12 h,差异表达基因有476条,其中上调的331条,下调的145条;照后24 h,差异表达基因有979条,其中上调的523条,下调的456条;共同差异表达的基因有112条。RT-PCR验证结果表明,EGR1和TRIAP1相对定量结果与基因芯片检验结果一致。对部分差异表达基因的生物信息学分析结果,发现了涉及到的主要途径,如细胞凋亡途径、p53依赖DNA损伤响应途径及死亡受体信号途径等。

The present study analyzed differential transcriptional profile of normal human lymphocyte and human lymphocyte radiated with 1.0 Gy gamma ray by using whole genome chip after 6,12,24 h point irradiation.RT-PCR was used to confirm the gene chip result.The results showed that there were 2615 differentially expressed genes after 6 hour irradiation,of which 1267 genes were up-regulated and 1348 genes were down-regulated.The results showed that there were 476 differentially expressed genes after 12 hour irradiation,of which 331 genes were up-regulated and 145 genes were down-regulated.The results showed that there were 979 differentially expressed genes after 24 hour irradiation,of which 523 genes were up-regulated and 456 genes were down-regulated.There were 112 co-expressed differential genes.RT-PCR results indicated that the relative quantity of result of EGR1 and TRIAP1 was consistent with gene chip data.The study indicated many significant pathways such as pathway of apoptosis,pathway of p53-Dependent G1 DNA damage response,pathway of death receptor signaling etc.