碘铁联合缺乏对大鼠血脂水平的影响

Effects of iodine and iron deficiency on serum lipid in rats

目的通过控制碘、铁摄入量，造成缺铁、缺碘模型，研究碘、铁缺乏对大鼠血脂水平的影响。方法将Sprauge—Dawley（SD）雄性大鼠随机分为4组：正常对照组（N组，饲料碘含量362．0μg/kg，铁含量93．3mg／kg），碘缺乏组（ID组，饲料碘含量61．4μg／kg，铁含量93．3mg／kg），铁缺乏组（FD组，饲料碘含量362．0μg／kg，铁含量9．6mg／kg），碘、铁联合缺乏组（IFD组，饲料碘含量61．4μg/kg，铁含量9．6mg／ks），每组15只。喂养6周后，检测尿碘水平，取血处死。分离血清，采用生化法检测血清甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、血红蛋白、血清铁水平和总铁结合力；化学发光法检测血清L、T4水平。结果IFD组和FD组血红蛋白水平[（69．2±7．4）g／L，（62．2±8．1）g／Lm（161．1±9．4）g/L，P〈0．01]和血清铁[（4．9±1．4）μmol／L，（5．2±0．8）μmoL／L郴．（27．3±6．5）μmol／L，P〈0．01]降低，总铁结合力[（176．3±4．4）μmol／L，（186．4±13．7）μmol／L vs ．（99．1±9．0）μmol／L，P〈0．01]升高，表现为中、重度贫血。与N组比较，血清T4水平在FD组[（47．42±6．58）nmol／L]、ID组[（35．27±6．97）nmol／L]和IFD组[（30．07±4．75）nmol／L]依次降低（P〈0．叭），但L水平在IFD组[（0．95±0．13）nmol／L]和FD组[（0．90±0．14）nmo]／L]降低，而在ID组[（1．46±0．14）nmol／L]升高（P〈0．01）。IFD组及FD组血清Tc[（1．77±0．30）mmol／L，（1．54±0．23）mmol／L]、HDL[（0．83±0．18）mmol／L，（0．59±0．10）mmol／L]和LDL[（0．67±0．24）mmol／L，（0．64±0．25）mmol／L]水平与N组比较均显著下降（P〈0．01）。ID组血清LDL[（0．79±0．17）mmol／L]水平与N组比较显著下降（P〈0．05）。结论碘、铁缺乏均可对血脂代谢产生影响，同时可相互作用，共同调节体内血脂水平。

Objective To investigate the effects of iodine and iron deficiency on lipid metabolism in rats by controlling the iodine and iron intakes ,making iodine deficiency and/or iron deficiency model. Methods Male Sprange-Dawley（SD） rats were randomly divided into 4 groups：normal control group （ N group ,iodine content was 362. 0 μg/kg and iron content was 93.3 mg/kg in the diet）,iodine deficiency group（ ID group,iodine content was 61.4μg/kg and iron content was 93.3 mg/kg in the diet）, iron deficiency group （FD group, iodine content was 362.0 μg/kg and iron content was 9.6 mg/kg in the diet ）, iodine and iron deficiency group （ IFD group, iodine content was 61.4μg/kg and iron content was 9.6 mg/kg in the diet ）, 15 in each group. After six weeks ,urinary iodine level was determined. Then the rats were killed by exsanguination and the blood was sampled to separate serum. The blood samples were analyzed for triglyceride （TG）, total cholesterol （TC）, high density lipopmtein （HDL）, low density lipoprotein （LDL）, hemoglobin, serum iron levels and total iron binding capacity with biochemical assay. Also,the levels of serum T3 and T4 were examined with chemiluminescence. Results In IFD group and FD group, the level of hemoglobin [（69.2±7.4）g/L,（62.2 ±8.1）g/L vs. （161.1 ±9.4）g/L, P〈 0. 01 ] and serum iron [ （4. 9 ± 1.4 ） μmol/L, （ 5.2 ± 0. 8 ） μmol/L vs. （ 27.3 ± 6.5 ） μmol/L, P 〈 0.01 ] were de creased, while the levels of total iron binding capacity [ （ 176. 3 ± 4.4） pjnolfL, （ 186.4 ± 13.7 ）μmol/L vs. （99.1 ±9.0）μmol/L, P 〈0. 011 were increased, which were defined as moderate to severe anemia. The levels of T4in FD group [（47. 42 ± 6. 58） nmol/Ll, ID group [（35. 27 ± 6. 97） nmol/L], and IFD group [ （30. 07 ± 4.75）nmol/L] were distinctively decreased compared with N group （P 〈 0. 01 ）, while the levels of T3 were decreased in IFD group [ （0.95 ±0. 13）nmol/L] and FD group[ （0.90 ±0. 14）nmol/L], and increased in ID group I （ 1.46 ± 0. 14） nmol/L ] compared with N group （P 〈 0.01 ）. The levels of TC [ （ 1.77 ± 0.30 ） retool/L, （1.54 ±0.23） mmol/L] ,I-IDLE （0.83 ±0. 18）mmol/L, （0. 59 ±0. 10） mmol/L] and LDL[ （0.67 ±0.24） mmol/L, （0. 64 ± 0.25 ）mmol/L] were decreased in IFD group and FD group compared with N group （P 〈 0. 01 ）. In ID group, the level of LDL [ （0. 79± 0. 17 ） mmol/L ] was decreased compared with N group （ P 〈 0. 05 ）. Conclusions Either iodine or iron deficiency can influence lipid metabolism. They can interact with each other, and play important roles in the metabolism of serum lipids.