二氧化钛负载细菌纤维素纳米复合材料的抗菌性及细胞相容性的研究

Study on Antibacterial Activity and Cell Compatibility of TiO_2/BC Nanocomposite

利用细菌纤维素的超精细网络结构和持水率高的特点,通过钛酸异丙酯水解在细菌纤维素上原位负载了TiO2颗粒,并对其微观结构等进行表征,同时对二氧化钛负载细菌纤维素纳米复合膜的抗菌性能和生物相容性进行研究。抗菌实验结果说明TiO2/BC纳米复合材料具有很强的抗菌性能,对大肠杆菌和金黄色葡萄球菌的抑菌圈直径分别达到15 mm和10 mm。细胞相容性实验结果显示,3T3细胞能够在TiO2/BC复合材料表面上正常生长、迁移铺展,这表明TiO2/BC纳米复合材料还具有良好的细胞相容性。因此基于该材料优良的抗菌性和生物相容性,将其应用于抗菌伤口敷料会有广阔的应用前景。

Titanium dioxide particle was prepared in situ by hydrolysis of isopropyl titanate and loaded on the surface of bacterial cellulose（BC） because BC possessed ultra-fine network and high water holding.Its microstructure was characteristed.The antibacterial activity and biocompatibility of TiO2/BC nanocomposites were studied.Antibacterial test results showed that TiO2/BC nanocomposites had strong antibacterial properties.Its diameters of antibacterial circle against escherichia coli and staphylococcus aureus reached 15 mm and 10 mm,respectively.Biocompatibility test results showed that TiO2/BC nanocomposites also had good biocompatibility.3T3 cells could grow and sprawl on the surface of TiO2/BC nanocomposites.Therefore,this material could be used as promising antibacterial biomaterial for would dressings based on its excellent antibacterial activity and biocompatibility.