紫外分光光度法测定甲砜霉素原料含量方法的建立

Determining the content of thiamphenicol in raw materials by ultraviolet spectrophotometry

采用紫外分光光度法测定甲砜霉素原料药的含量。实验结果表明,甲砜霉素的最大吸收波长为228 nm,在此波长处,甲砜霉素在5.0～20.0μg/mL浓度范围内,其吸光度与浓度呈现良好的线性关系:A=0.0381C-0.1074,R2=0.9992(n=7)。该方法操作简单、快速,结果准确,可用于甲砜霉素原料药的含量测定。

The content of thiamphenicol in raw materials was determined by the method of ultraviolet spectrophometry. The results showed that the maximum absorption wavelength of thiamphenicol was 228nm and the linear relation between the absorbency and the concentration was good when the concentration of thiamphenicol was between 5.0 and 20.0μ g/mL： A=0.0381C-0.1074, RZ=0.9992 （n=7） . This method was simple and quick, and the results was reliable. It could be applied to quick determination of thiamphenicol in the raw materials later.