野生兰属植物根部内生真菌多样性初步研究

Preliminary Study on Diversity of Endophytic Fungi from Wild Cymbidium Plants Roots(Orchidaceae)

采集生长在我国广西、贵州、云南的密林、开阔地两种生境的9种兰属植物,对其根部的内生真菌进行分离,经过形态观察和ITS序列测定相结合的鉴定。结果表明,分离得到的99个菌株隶属于29个菌属。对子囊菌和担子菌分别进行的系统发育树构建结果显示,子囊菌为优势菌种,其中镰刀菌属(Fusarium,22.20%),炭角菌属(Xylaria,6.06%)和木霉属(Trichoderma,5.05%)为优势菌属。物种丰富度和Simpson多样性指数(D)分析结果表明,密林生境的兰属植物的菌根真菌多样性高于开阔生境。

The nine species of Cymbidium plants were collected from Guanxi,Guizhou and Yunnan provinces,the endophytic fungi of their root were isolated,its patterns were observed and its sequences were determined.The results showed that the isolated 99 endophytic fungi strains belong to 29 genera.The structure results of phylogenetic dentrogram produced by Ascomycota and basidiomycete showed that Ascomycota was more dominant than basidiomycete.Among them Fusarium（22.20%）,Xylaria（6.06%） and Trichoderma（5.05%） of Ascomycota were the dominant fungi Species number and Simpson diversity index（D） of endophtic fungi from the orchids growing in forest habitat were higher than those in the habitat of openness.