BAF反应器中氨化细菌的筛选与氨化特性分析

Isolation of amonifying bacteria from BAF reactor and analysis on amination characterization

以BAF反应器中的生物膜为接种物,分离筛选得到2株氨化细菌,分别命名为A1、A2。通过形态学特征观察、生理生化特性分析及氨化性能进行表征,初步鉴定A1为变形杆菌属(Proteus),A2为微球菌属(Micrococcus)。生态影响因子实验确定菌株氨化过程主要影响因素是温度、初始pH、投菌量。在30℃、初始pH值7.0,接种量5%的最佳运行条件下,A1、A2两株氨化细菌对有机氮分解效率分别达到86.91%、69.98%。所分离的2株氨化细菌对于高浓度有机氮工业废水的生物降解有一定的应用价值。

Two bacterial strains A1 and A2 screened from the biofilm of BAF reactor were found capable of ammonification. By the morphologic observation, physiological biochemistry characteristic analysis and amination performance characterization, the strains A1 and A2 were preliminarily identified as Proteus and Micrococcus, re- spectively. On the basis of analyzing the impact factors, it was found that the main factors affecting ammonifica- tion by strains A1 and A2 were temperature, initial pH and bacteria dosage. When the temperature was 30 ℃, the pH value was 7.0 and the bacteria dosage was 5% , the degradation of organic nitrogen of two strains A1 and A2 could reach up to 86.91% and 69.98% , respectively. The two strains A1 and A2 had a certainly practical value for treatment of the high concentration of organic nitrogen in wastewater.