鸡冠花炭指纹图谱初步研究

Study on the HPLC Fingerprint of Celosia Cristata Carbonisata

目的建立鸡冠花炭的HPLC指纹图谱分析方法。方法采用HPLC法测定10批鸡冠花炭样品,采用《中药色谱指纹图谱相似度评价系统(2004A)版》,建立了共有模式,以相关度评价图谱的相似性,以共有峰与对照峰的比值对各鸡冠花炭样品进行聚类分析。结果 10批次鸡冠花炭样品色谱指纹图谱共有10个共有峰,相似度在0.89～0.99,可通过系统聚类归为一类,不同批次鸡冠花炭质量相似性较好。结论该方法可用于鸡冠花炭质量评价。

OBJECTIVE To establish the method to analyze HPLC fingerprint of Celosia Cristata L.Carbonisata.METHODS Chromatographic fingerprint of Celosia Cristata L.Carbonisata was investigated by RP-HPLC and the gradient elution mode was applied to chromatographic separation.Data was analyzed by fingerprint similarity evaluation software to compare the similarity of the samples for identifying the main chromatographic peaks furthermore.Samples were clustered with principal component analysis（PCA） based on the ratio of characteristic peaks and standard peaks.RESULTS The chromatographic fingerprint of 10 batches of Celosia Cristata L.Carbonisata showed 10 characteristic peaks,with the similarity from 0.89 to 0.99,and the samples can be classified into one group based on the hierarchical cluster.The qualities of Celosia Cristata L.Carbonisata in different batches were in a good similarity.CONCLUSION The method can be applied to assess the quality of Celosia Cristata L.Carbonisata.