人巨细胞病毒活动性感染与习惯性流产的关系探讨

Relationship between HCMV active infection and habitual abortion

目的：探讨人巨细胞病毒（HCMV）活动性感染与习惯性流产的关系。方法：选择2008年5月。2010年5月我院妇产科收治的反复性流产孕妇40例作为研究组，选择同期体检正常孕妇50例作为对照组，采集两组患者外周血，离心分离出有核细胞及血浆，采用实时荧光定量PCR和免疫荧光法检测HCMV-DNA和HCMVpp65抗原，分析HCMVpp65抗原阳性与流产的关系及两种检测方法的一致性。结果：研究组HCMVpp65抗原阳性率明显高于对照组，两组比较，差异有统计学意义（X2=7．69，P〈0．05）；研究组中流产次数多的孕妇，HCMVpp65抗原阳性率明显高于流产次数相对较少的孕妇，差异有统计学意义（x2=8．58，P〈0．05）。研究组中实时荧光定量PCR检测HCMV—DNA和免疫荧光法检测HCMVpp65抗原的一致性较好，达92．5％。结论：孕妇习惯性流产与其体内巨细胞病毒活动性感染有重要联系．习惯性流产孕妇HCMVpp65抗原检测与HCMV—DNA有良好的一致性，可作为习惯性流产的早期判断指标。

Objective： To investigate the relationship between human cytomegalovirus （HCMV） active infection and recur- rent spontaneous abortion （RSA）. Methods： 40 cases of RSA pregnant women in our hospital from May 2008 to May 2010 were selected as study group, and 50 cases of normal pregnant women were selected as control group. Peripheral blood of two groups were collected, mononuelear cells and plasma were centrifuged, then HCMV-DNA and HCMVpp65 antigen were detected by real-time PCR and immunofluorescence. The relationship between positive HCMVpp65 antigen and abor- tion, and the consistency of the two detection methods were analyzed. Results： The positive rate of HCMVpp65 antigen of study group was significantly higher than that of control group, the difference was statistically significant （X2=7.69, P〈0.05）; the positive rate of HCMVpp65 antigen of pregnant women with more times abortions in study group was significantly high- er than that of the pregnant women with less times abortion, the difference was statistically significant （X2=8.58, P〈0.05）; in study group, the consistency of HCMV-DNA and HCMVpp65 antigen detection is high, with the rate of 92.5%. Conclu- sion： RSA of pregnant women has important relationship with active infection of HCMV. HCMVpp65 antigen detection has good consistency with HCMV-DNA, it can be used as an early determine indicators of abortion.