基于反相液相色谱的人血中茶碱浓度临床检测与分析

Detection of theophylline in human serum and its clinical application based on reversed-phase HPLC

目的:建立快速、简便、准确、重现性好的人血中茶碱的测定方法。方法:采用Hypersil ODS C18(4.6 mm×250 mm,5μm)为色谱柱,流动相为甲醇-水(25∶75),内标物为咖啡因,检测波长为274 nm,血清用甲醇沉淀蛋白后进行分析。结果:茶碱标准曲线回归方程为Y=0.053 9X+0.038,r=0.999 4(n=8),1～40μg/ml浓度范围线性关系良好。对36例患者作血清茶碱浓度监测,临床效果良好。结论:本测定方法为临床药师指导临床有效、安全的个体化药物的使用提供了保障。

Objective： To develop a rapid, simple, accurate and reproducible method for the determination of theophylline in human serum. Methods： The separation was performed on Hypersil ODS C18 column （4.6 mm×250 mm, 5 μm） with mo- bile phase consisted of methanol-water （25：75）, and the wavelength for detection was 274 nm. The coffeine was used as an internal standard, and methanol was used to precipitate the serum protein. Results： The regression equation of theophylline was Y=0.053 9X＋0.038, r=-0.999 4 （n=8）. The calibration curves of theophylline were linear over the range of 1-40 μg/ml. The clinical application was good for 36 patients through of the detection of theophylline. Conclusion： The method sup- plies a reasonable guide for the drug use at individual levels.