宫颈鳞状细胞癌及癌前病变中FHIT、PCNA的表达及意义

Expression and clinical significance of FHIT and PCNA protein in Cervical Squamous Cell Carcinoma and Cervical Intraepithelial Neoplasia

目的:探讨FHIT、PCNA在宫颈鳞状细胞癌及癌前病变中的表达规律,研究其在宫颈癌变中的作用和意义。方法:采用免疫组织化学(S-P法)检测FHIT、PCNA在18例正常宫颈组织、52例CIN和69例宫颈癌中的表达。结果:FHIT在正常宫颈组织、CINⅠ、CINⅡ、CINⅢ和宫颈鳞癌组织中阴性率分别为0%、9.1%、29.4%、41.7%、66.7%,正常组与宫颈癌组之间、CIN组与宫颈癌组之间、CINⅠ、Ⅱ组与CINⅢ组之间比较,差异均有显著性(P<0.05);随着细胞分化的降低,FHIT阴性率上升。PCNA在宫颈癌中的阳性率为84.1%,高于CIN和正常组(P<0.05),CIN和宫颈癌两组FHIT阴性者的PCNA阳性率明显高于FHIT正常表达者(P<0.01),FHIT与PCNA呈负相关关系。结论:FHIT、PCNA蛋白异常表达与宫颈癌的发生密切相关,FHIT缺失可作为判断CIN有可能发展为浸润癌的有用指标。CIN和宫颈鳞癌中的FHIT缺失常与PCNA过度表达同时存在,两者存在负相关关系。

Objective：To study the expression of FHIT and PCNA in cervical squamous Cell carcinoma（SCC） and cervical intraepithelial neoplasia（CIN） and to investigate the role and significance of them.Methods：The expression of FHIT protein and PCNA protein was determined using immunohistochemistry（S-P） in 18 cases of normal cervical squamous epithelium,52 cases of CIN and 69 cases of cervical SCC.Results：The rates for loss of expression of FHIT genes in the squamous epithelium of normal cervices,CIN Ⅰ,CIN Ⅱ,CIN Ⅲand invasive cervical SCC were 0%,9.1%,29.4%,41.7%,66.7%.The comparison between normal cervical squamous epithelium（NEC） and cervical SCC,the comparison between CIN and cervical SCC,the comparison between CIN Ⅰ、Ⅱ and CIN Ⅲ have statistic significance（P0.05）.Along with the decreasing of cell differentiation,the negative rate of FHIT raised.The positive expression of PCNA in cervical SCC group was 84.1%,higher than that in CIN and normal groups（P0.01）.The positive rate of PCNA in the depleted expression of FHIT group was significantly higher than that in FHIT normal expression group（P0.01）.There is a negative correlation between FHIT And PCNA expression.Conclusion：The FHIT-depletion and PCNA over-expression are related with cervical carcinogenesis.The FHIT-depletion may be a clinically useful marker of CIN more likely to progress to invasive disease;Depleted expression of FHIT is freguently associated with PCNA over-epression in CIN and cervical SCC subjects.There is a negative correlation between FHIT And PCNA expression.