摘要
为了建立适合空间诱变辣椒的AFLP反应体系,以3～4叶期的辣椒叶片为试材,对体系中的DNA用量、酶切与连接、预扩增及产物的稀释倍数等关键因素进行了优化,确立了适合空间诱变辣椒的AFLP反应体系。结果表明:采用该体系对空间诱变辣椒进行AFLP分析,能够得到清晰稳定的分子标记图谱。
In order to establish a suitable AFLP reaction system of pepper induced by space,taking 3~4 leaf stage of pepper leaves as test materials,several significant influencing factors,including the dosage of template DNA,enzyme digestion and ligation,pre-amplification and the dilute of pre-amplification products were optimized,and a suitable AFLP reaction system for hot pepper induced by space was constructed.The results showed that by this optimized system,a clear and credible AFLP fingerprint could be obtained.
出处
《北方园艺》
CAS
北大核心
2011年第21期103-105,共3页
Northern Horticulture
基金
航天育种工程资助项目(2006HT20012)
国家大宗蔬菜产业技术体系资助项目(CARS-25-G-11)
黑龙江省自然科学基金重点资助项目(ZD200818-01)
黑龙江省自然科学基金资助项目(C200943)
关键词
空间诱变
辣椒
AFLP反应体系
优化
induced by space
hot pepper
AFLP analysis system
optimization
