常见观赏竹类过氧化物酶同工酶及可溶性蛋白分析

Analysis Peroxidase Isozyme and Soluble Protein in Ornamental Bamboos

为了探讨常见观赏竹类的亲缘关系,采用聚丙烯酰胺凝胶电泳(PAGE)技术分析了竹类植物过氧化物酶同工酶和可溶性蛋白。结果表明:供试材料的过氧化物酶同工酶和可溶性蛋白的位点和数目均存在差异。相似性系数在0.400～0.762,同一种属的金边富贵竹与青叶富贵竹相似性系数最高(0.762)。聚类结果显示,6个供试样品聚成二大类,与形态分类结果基本一致。试验结果可以初步作出常见观赏竹类品种间亲缘关系的判定。

In order to research the genetic differences of ornamental bamboos,the peroxidase isozyme of bamboo leaves were analyzed by plumb plate of polyarclamide gel electrophoresis.The results showed that there were certain differences in the number and locus of the peroxidase isozyme and soluble protein bands among the accessions.The similarity coefficient was 0.400~0.762.The similarity coefficient between the two varieties of Dracaena sanderiana was the highest（0.762）.The clustering analysis indicated that all samples were divided into 2 Groups,which was in accordance with the morphological taxonomy of bamboos.The study showed that ornamental bamboos could be distinguished by peroxidase isozyme and soluble protein analysis.