罗布麻嫩茎诱导分化培养基的筛选

Screening of Differentiation Medium of Tender Bine from Apocynum venetum L.

以罗布麻无菌嫩茎尖为外植体,以MS为基本培养基,添加不同浓度的NAA、2,4-D,研究不同培养基组合对罗布麻诱导分化成活率的影响。结果表明:罗布麻嫩茎诱导分化最佳培养基组合为MS+NAA 0.01mg/L+2,4-D 0.5mg/L,诱导成活率为73.3%。

Taking Tender stem tip from Apocynum venetum L.as explant and MS as basic medium,adding different concentration of NAA and 2,4-D,the effect of different media combination on differentiation survival rate of Apocynum venetum L.were studied.The results showed that the best medium combination for tender bine differentiation of Apocynum venetum L.was MS＋NAA 0.01 mg/L＋2,4-D 0.5 mg/L,survival rate was 73.3%.