雨生红球藻异养转化产虾青素的条件研究

Study on astaxanthin accumulation by heterotrophic transformation in Haematococcus pluvialis

对比自养转化与异养转化的虾青素产量,发现向培养基中添加乙酸钠进行异养转化可使羽生红球藻的虾青素产量由0.723 6 mg·L-1提高到1.216 8 mg·L-1;为进一步提高虾青素产量,在单因素实验基础上,选择乙酸钠浓度和硝酸钾浓度为自变量,虾青素产量为响应值,利用中心组合设计实验和响应面分析法,对转化培养基进行优化.结果表明:异养转化培养基中的乙酸钠和硝酸钾浓度对虾青素产量影响较大,在乙酸钠1.460 5 g·L-1,硝酸钾0.008 4 g·L-1,磷酸二氢钾0.02 g·L-1时,虾青素产量最高,达10.908 mg·L-1,与对照相比提高了10.16倍,与自养转化相比提高了14.07倍.

The effects of heterotrophic transformation and autotrophic transformation on astaxanthin production in Haematococcus pluvialis were studied and compared.When sodium acetate was added in MCM for heterotrophic transformation,astaxanthin accumulation was increased from 0.7236 mg·L-1 to 1.2168 mg·L-1.To enhance the astaxanthin accumulation,single factor experiment was founded up.Center composite design experiments and response surface methodology were employed to optimize the media of heterotrophic transformation.The results showed a distinctive influence of sodium acetate and potassium nitrate concentrations on astaxanthin accumulation.The optimal fermentation medium consisted of 1.4605 g·L-1 sodium acetate,0.0084 g·L-1 potassium nitrate,and 0.02 g·L-1 potassium dihydrogen phosphate.Astaxanthin content reached 10.908 mg·L-1,which was raised 10.16-fold against the control group and 14.07-fold against the group of autotrophic transformation.