浙闽香鱼4个群体遗传多样性的RAPD分析

RAPD Analysis on Genetic Diversity of Four Populations of Plecoglossus altivelis from Zhejiang and Fujian Province

采用RAPD分子标记技术对采自福建东张水库(DZ)、浙江楠溪江(N8、N9)和浙江省海洋水产养殖研究所清江试验场(QJ)的香鱼4个群体进行遗传多样性和遗传关系分析。筛选出15条RAPD引物在4个群体72个样品中共检测到106个稳定清晰的位点,其中多态性位点47个,多态位点比例(PPL)为44.34%。群体遗传多样性分析结果表明,香鱼4个群体的多态性位点比例为23.58%～35.85%,Nei’s基因多样性指数(H)和Shannon信息指数(I)分别在0.0868～0.1410和0.1285～0.2049之间,表明其遗传多样性水平不高,应加大对香鱼种质资源的保护力度。根据群体间遗传距离指数,UPMG方法聚类分析显示,N8和N9香鱼群体亲缘关系最近,首先聚为一支,DZ群体和QJ群体聚类在另一分支。

Genetic diversity and genetic structure of four populations of Plecoglossus altivelis collected from Dongzhang Reservoir of Fujian province（DZ）,Nanxijiang River of Zhejiang province（N8 and N9） and Qingjiang test field of Zhejiang Mariculture Research Institute（QJ）,were analyzed by random amplified polymorphic DNA（RAPD）.A total of 106 stable and distinct bands,including 47 polymorphic ones,were detected from the four populations by using 15 primers.The percentage of polymorphic locus was 44.34%.The results of data analysis on four populations showed that the value of percentage of polymorphic locus,Nei＇s gene diversity and Shannon＇s information index were 23.58%～35.85%,0.0868～0.1410 and 0.1285～0.2049 respectively.These results showed that their genetic diversity were low,we should strengthen the protection of the Plecoglossus altivelis resources.Clustering analysis by UPMG method showed N8 and N9 priority clustered together,while DZ and QJ cultured clustered in another branch.