制备Phosvitin时蛋黄水溶物去除工艺研究

Optimizating Process Conditions of Removing Soluble Substance from Yolk during Phosvitin Preparation

卵黄高磷蛋白存在于蛋黄非水溶性的物质部分,通过单因素试验和正交试验优化了蛋黄中提取卵黄高磷蛋白的除去蛋黄中水溶性蛋白工艺条件。结果表明:水溶性和非水溶性物质的分离条件为HAc-NaAc缓冲液(0.02 mol/L,pH5.0),料液比1∶6(g/mL),搅拌时间30 min,洗涤次数为3。

Phosvitin is a kind of yolk protein and exists in non-water soluble part of yolk.The isolation conditions for water-soluble and non-water-soluble yolk parts were optimized in orthogonal experiment together with single effective factor analysis.Results shows that： HAc-NaAc buffer（0.02 mol/L,pH=5.0）,ratio of material to exaction liquid being 1∶6（g/mL）,extraction time being 30 min,and turns being 3.