摘要
用HPLC法测定中国被毛孢菌丝体中的5种核苷的含量。采用Hypersil GOLD AQ色谱柱（4.6 mm×250 mm,5μm）,甲醇：0.01 mol/L：KH2PO4=5∶95（体积比）为流动相,流速1 mL/min,检测波长260 nm。结果发现,液体发酵和静置培养的中国被毛孢菌丝体中胞苷含量分别为266.73μg/g和348.79μg/g,尿苷分别为1 718.23μg/g和1 558.99μg/g,肌苷分别为892.64μg/g和573.31μg/g,鸟苷分别为1 370.78μg/g和1 444.30μg/g,腺苷分别为1 296.68μg/g和1 440.83μg/g。5种核苷测定的精密度RSD值介于0.97%~3.75%间、稳定性RSD值范围为2.91%~3.86%、重现性RSD值范围为1.28%~2.91%、回收率范围为91.07%~102.21%。
A HPLC method was used to detect 5 nucleoside contents of liquid fermented Hirsutella sinensis mycelium.The HPLC conditions were as Hypersil GOLD AQ column（4.6 mm×250 mm,5 μm）,methanol： 0.01 mol/L KH2PO4（5∶95,volume ratio） as a mobile phase and peaks were monitored at 260 nm.The results showed that the contents of nucleoside in liquid fermented or liquid still cultured mycelia were 266.73 μg/g and 348.79 μg/g（cytidine）,1 718.23 μg/g and 1 558.99 μg/g（uridine）,892.64 μg/g and 573.31 μg/g（inosine）,1 370.78 μg/g and 1 444.30 μg/g（guanosine）,and 1 296.68 μg/g and 1 440.83 μg/g（adenosine）,respectively.The RSDs of accuracy,stability and reproducibility were in between 0.97 %-3.75 %,2.91 %-3.86 % and 1.28 %-2.91 %,respectively.The recoveries were in between 91.07 %-102.21 %.
出处
《食品研究与开发》
CAS
北大核心
2011年第11期96-99,共4页
Food Research and Development
