HCVRNA定量及HCV核心抗原和丙氨酸氨基转移酶结果分析

Analysis on the detection results of HCV RNA,HCV core antigen and alanine ammotrans ferase

目的分析丙型肝炎病毒(HCV)RNA定量检测结果与HCV核心抗原及丙氨酸氨基转移酶(ALT)检测结果的相关性。方法收集涪陵监狱卫生所94例住院和门诊患者血清,采用逆转录聚合酶链反应荧光探针法定量检测标本中的HCV RNA,同时采用酶联免疫吸附试验检测HCV核心抗原,采用全自动生化分析仪检测ALT水平。结果 94例样本中HCV RNA阳性率为56.4%(53/94),HCV核心抗原阳性率为53.2%(50/94),经统计学分析,两种方法的阳性率差异无统计学意义(P>0.05),符合率为84.9%(45/53);ALT异常率为62.3%(33/53),随着HCV RNA含量的升高而增加。结论 HCV RNA定量检测结合HCV核心抗原及ALT检测,可帮助临床了解HCV在体内的复制水平及肝脏的炎性反应状态,以指导临床用药及疗效观察。

Objective To investigate the relativity of quantitative detection of HCV RNA and detection of hepatitis C virus（HCV） core antigen and alanine ammotrans ferase（ALT）.Methods 94 human serum were collected from inpatients and outpatients.HCV RNA was quantitatively detected by real-time fluorescence PCR.HCV core antigen was measured by enzyme-linked immunosorbent assay（ELISA）,and ALT was detected by using automatic biochemical analyzer.Results The positive rates of HCV RNA and HCV core antigen were 56.9%（53/94） and 53.2%（50/94）,respectively.There was no significant statistical difference between two methods（P0.05） and the coincidence rate was 84.9%（45/53）.The abnormal rate of ALT was 62.3%（33/53）,which was enhanced with the increase of HCV RNA.Conclusion Quantitative detection of HCV RNA and HCV core antigen test with ALT results can help clinicians to understand the level of HCV replication in the body and the inflammatory response status of the liver.This will guide clinical treatment and observation of treatment effects.