摘要
目的:对大豆异黄酮降低胆固醇作用及其机制进行探讨,并比较金雀异黄素(Gen),大豆素(Dai)和雌马酚(Eq)的作用强弱。方法:采用MTT检测细胞增殖状况,采用试剂盒检测LDL-C、TC和apoA-I的表达,采用实时定量PCR检测mRNA的表达。结果:大豆异黄酮可以增加细胞内胆固醇含量,增加LDL-C的摄取量和apoA-I的分泌量,其中Gen的作用最为明显,Eq的作用较弱。PPARγ和LXRα的mRNA表达量增多。结论:大豆异黄酮能够调节细胞胆固醇代谢,其作用可能是通过调节PPARγ/LXRα通路实现的。
【Objective】To explore the effect of soy isoflavones on cholesterol metabolism in HepG2 cells and the possible mechanism of it and to compare the strength of Gen,Dai,and Eq.【Method】The proliferation of HepG2 cell was by MTT,the measurement of LDL-C and TC content was by the kit,the apoA-I content was by Elisa kit,and the measurement of mRNA was by real time PCR.【Result】Soy isoflavones could increase the cellular cholesterol content,LDL-C uptake and apoA-I secretion.Gen had the strongest effect and Eq had the weakest.The mRNA expression of PPARγ and LXRα was increased.【Conclusion】Soy isoflavones could affect cholesterol metabolism and it was maybe through the PPARγ/LXRα pathway.
出处
《中国食物与营养》
2011年第11期66-69,共4页
Food and Nutrition in China
基金
国家自然科学基金(项目编号:30671759
30872114
30872115)
