多菌种混合发酵棉籽饼粕制备益生菌生物活性饲料

Multi-strain mixture of the fermented cottonseed meal for the preparation of probiotics biological activity feed

以棉籽饼粕、麸皮、苹果渣为原料,经霉菌XH001、霉菌XH002、地衣芽孢杆菌E01、枯草芽孢杆菌E02、乳酸菌SR01、酿酒酵母和饲料酵母混合发酵生产益生菌饲料。最佳工艺条件为:发酵温度32℃,原料含水量50%,芽孢杆菌接入时间为发酵后12 h,乳酸菌接种量10%,发酵时间48 h。发酵后饲料粗蛋白含量由原来的27.58%提高到37.92%,氨基酸态氮含量达到1.88%,乳酸菌活菌数达2.3亿cfu/g(干基),芽孢杆菌达18.4亿cfu/g(干基)。

With cottonseed meal,wheat bran,apple pomace as raw materials,the mold XH001,mold XH002,Bacillus licheniformis E01,Bacillus subtilis E02,lactic acid bacteria SR01,feed yeast saccha-romyces cerevisiae and the production of probiotic fermented were mixed for producing probiotics feed.Optimum conditions： fermentation temperature was 32 ℃,water content of raw materials was 50%,access time for the fermentation of bacillus was in 12 h,lactic acid bacteria inoculum was 10%,fermentation time was 48 h.After the fermentation,the feed crude protein content was up from 27.58% to 37.92%,amino nitrogen content was 1.88%,amounting to 230 million cfu/g（dry basis） viable lactic acid bacteria,and 1.84 billion cfu/g（dry basis）bacillus.